Effect of hyperoxia on articular tissues in organ culture.

Pig articular cartilage in organ culture. Effect of enzymatic depletion of the matrix on response of chondrocytes to complement-sufficient antiserum against pig erythrocytes.

The comparison of the effect of an aerobic training period and ozone therapy on gene expression of Beclin-1, AMPK and mTOR of articular cartilage tissue in rats model of osteoarthritis

Introduction The aim of the research was to investigate the effect of 8 weeks’ exercise training and ozone therapy on articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression in rat’s model of osteoarthritis (OA). Materials and Methods In this experimental study, 40 adult male rats 210±10 gr were randomly divided into 5 groups: 1) control (CN), 2) OA, 3) OA + ozone, 4) OA + ex...

Study of Expression Level of Cartilage Genes in Rat Articular Chondrocyte Monolayer and 3D Cultures using Real Time PCR

Purpose: to compare the expression level of certain genes related to cartilage and non-cartilage tissues at monolayer and alginate cultures derived from rat articular cartilage. Materials and Methods: Articular cartilage was harvested from knee joints of 10 male rats and was digested using enzymatic solution consisting of 0.2% collagenase I and 0.1% pronase. Released chondrocyte were then plate...

گزارش یک مورد آلکاپتونوری (Alkaptonuria)

Âlkaptonuria is a very rare hereditary disease with the liver enzyme homogentisic oxidase deficiency which is the result of accumulation of homogentisic acid in tissues along with excretion in urine in large quantities. Ôchronosis is clinical expression of alkaptonuria with the symptoms on the visceral organ, articular and connective tissues. The patient was a middle-aged man with prolo...

Mesenchymal Stem Cell Purification from the Articular Cartilage Cell Culture

Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Artic...